Journal: Molecular neurobiology

Article Title: Intracerebral expression of AAV-APOE4 is not sufficient to alter tau burden in two distinct models of tauopathy

doi: 10.1007/s12035-019-01859-4

Figure Lengend Snippet: Neonatal PS19 mice were injected with AAV-APOE4 or AAV-EGFP and aged to 3 months of age. APOE4 expression does not alter human tau transgene, as shown by CP27-immunoreactive total human tau (A). Quantification of tau protein band normalized to GAPDH loading control is shown (B). n=5 mice/group. Unpaired t test. Representative images of CP13 immunoreactivity from 3 month old mice are shown from different regions of the cortex and hippocampal CA1 (C). Scale bar: 100 μm. Quantitation of the immunostaining is presented as % immunoreactivity in the cortex and hippocampus (D). n= 5–11 mice/group. Two way ANOVA. Western blotting with PBS extracted brain lysate (denoted as S1) was done using ptau antibody (CP13, E-F) and total mouse tau antibody (T49, G-H) with protein band normalized to Gapdh loading control (F, H). n=5 mice/group. Unpaired t test. Pretangle tau levels were assessed by MC1 immunostaining in different areas of the cortex and hippocampus CA1 area (I) and data quantified as % immunoreactivity (J). Scale bar: 100 μm. n=5–11 mice/group. Two way ANOVA. Sarkosyl-insoluble cellular fraction (denoted as S4) was assessed for the presence of total human tau (K-L) and total mouse tau (M). n=5 mice/group. Unpaired t test. Black symbols, male mice; grey symbols, female mice.

Article Snippet: Statistical comparisons were conducted using unpaired t tests or 2 Way ANOVA with a Sidak post hoc test, if necessary (GraphPad Prism 7).

Techniques: Injection, Expressing, Control, Quantitation Assay, Immunostaining, Western Blot

Journal: Molecular neurobiology

Article Title: Intracerebral expression of AAV-APOE4 is not sufficient to alter tau burden in two distinct models of tauopathy

doi: 10.1007/s12035-019-01859-4

Figure Lengend Snippet: Morphometric analysis of the forebrain was done by counting hippocampal CA1–3 neurons and by measuring cortical thickness (frontal cortex, motor/sensory cortex, and auditory cortex) from specific brain areas of 4 month old rTg4510 mice (A-C) or 6 month old rTg4510 mice (J-L). This specific area was bounded by Bregma coordinates: 0.84mm and 1.08mm. Scale bar: 300 μm (larger panels on the left); 125μm (smaller panels on the right). n=4–6 mice/group. Unpaired t test of 2 way ANOVA. Synaptic protein profile was analyzed by immunoblotting for synaptogyrin 3, synaptophysin, spinophilin, PSD95, vGlut1 and GluR1 in the 4 month rTg4510 mice (D-I) and in the 6 month rTg4510 mice (M-R). Indicated protein bands were quantified following normalization to either GAPDH or Actin as shown. n=6/group (4 month rTg4510) and n=4 (6 month rTg4510). Black symbols, male mice; grey symbols, female mice.

Article Snippet: Statistical comparisons were conducted using unpaired t tests or 2 Way ANOVA with a Sidak post hoc test, if necessary (GraphPad Prism 7).

Techniques: Western Blot